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Monstervoorbereiding en relatieve kwantificering met behulp van reductieve methylering van amines voor peptidomics-studies
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Biochemistry
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JoVE Journal Biochemistry
Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
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Monstervoorbereiding en relatieve kwantificering met behulp van reductieve methylering van amines voor peptidomics-studies

Nederlands

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2,093 Views

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08:00 min

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November 04, 2021

DOI:

10.3791/62971-v

DOI:
10.3791/62971-v

•

08:00 min
November 04, 2021

•

10 Views
, , ,
1Department of Biological and Environmental Sciences, Bioscience Institute,Sao Paulo State University (UNESP), 2Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute,Sao Paulo State University (UNESP)

Summary

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Dit artikel beschrijft een monstervoorbereidingsmethode op basis van warmte-inactivatie om endogene peptiden te behouden die post-mortem degradatie voorkomen, gevolgd door relatieve kwantificering met behulp van isotopische etikettering plus LC-MS.

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Cite this Article

Correa, C. N., Fiametti, L. O., Mazzi Esquinca, M. E., Castro, L. M. d. Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies. J. Vis. Exp. (177), e62971, doi:10.3791/62971 (2021).

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