Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Claudia Neves Correa; Louise Oliveira Fiametti; Maria Eduarda Mazzi Esquinca; Leandro  Mantovani de Castro

doi:10.3791/62971

Journal

/

Bioquímica

/

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

JoVE Journal
Bioquímica

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Bioquímica

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

2,085 Views

•

08:00 min

•

November 04, 2021

DOI:

10.3791/62971-v

DOI:

10.3791/62971-v

•

08:00 min

November 04, 2021

•

10 Views

Claudia Neves Correa^1,2, Louise Oliveira Fiametti¹, Maria Eduarda Mazzi Esquinca¹, Leandro Mantovani de Castro^1,2

¹Department of Biological and Environmental Sciences, Bioscience Institute,Sao Paulo State University (UNESP), ²Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute,Sao Paulo State University (UNESP)

Summary

This article describes a sample preparation method based on heat-inactivation to preserve endogenous peptides avoiding degradation post-mortem, followed by relative quantitation using isotopic labeling plus LC-MS.

Explore More Videos

Sample Preparation Relative Quantitation Reductive Methylation Amines Peptidomics Protease Inactivation Isotopic Labeling Neuroblastoma Cells Zebrafish Brain Cellular Lysate Ultrafiltration Solid-phase Extraction

Read Article

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Summary

Automatically generated

Explore More Videos