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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

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08:00 min

November 04, 2021

DOI:

08:00 min
November 04, 2021

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Summary

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This article describes a sample preparation method based on heat-inactivation to preserve endogenous peptides avoiding degradation post-mortem, followed by relative quantitation using isotopic labeling plus LC-MS.

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