Journal
/
Biochimie
/
Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

DOI:
10.3791/62971-v

•

08:00 min

•

November 04, 2021

•

, , ,
1Department of Biological and Environmental Sciences, Bioscience Institute,Sao Paulo State University (UNESP), 2Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute,Sao Paulo State University (UNESP)

Chapitres

  • 00:04Introduction
  • 00:44Peptide Extraction: Cell Culture
  • 01:30Peptide Extraction: Animal Tissues
  • 03:39Peptide Quantification with Fluorescamine
  • 04:18Reductive Methylation of Amines Labeling
  • 05:58Results: Determining Labelled Peptides Using Mass Spectroscopy
  • 07:19Conclusion

Summary

Traduction automatique

Traduction automatique

This article describes a sample preparation method based on heat-inactivation to preserve endogenous peptides avoiding degradation post-mortem, followed by relative quantitation using isotopic labeling plus LC-MS.

Tags

Sample PreparationRelative QuantitationReductive MethylationAminesPeptidomicsProtease InactivationIsotopic LabelingNeuroblastoma CellsZebrafish BrainCellular LysateUltrafiltrationSolid-phase Extraction

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

Preparation of High-Temperature Sample Grids for Cryo-EM

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Read Article