Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Claudia Neves Correa; Louise Oliveira Fiametti; Maria Eduarda Mazzi Esquinca; Leandro  Mantovani de Castro

doi:10.3791/62971

Journal

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Biochimie

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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

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Biochimie

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Journal JoVE Biochimie

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

2,093 Views

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08:00 min

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November 04, 2021

DOI:

10.3791/62971-v

DOI:

10.3791/62971-v

•

08:00 min

November 04, 2021

•

10 Views

Claudia Neves Correa^1,2, Louise Oliveira Fiametti¹, Maria Eduarda Mazzi Esquinca¹, Leandro Mantovani de Castro^1,2

¹Department of Biological and Environmental Sciences, Bioscience Institute,Sao Paulo State University (UNESP), ²Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute,Sao Paulo State University (UNESP)

Summary

This article describes a sample preparation method based on heat-inactivation to preserve endogenous peptides avoiding degradation post-mortem, followed by relative quantitation using isotopic labeling plus LC-MS.

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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

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