Journal
/
Biochemistry
/
使用胺还原甲基化进行样品制备和相对定量，用于肽学研究
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
Please note that all translations are automatically generated. Click here for the English version.

使用胺还原甲基化进行样品制备和相对定量，用于肽学研究

DOI:
10.3791/62971-v

•

08:00 min

•

November 04, 2021

•

, , ,
1Department of Biological and Environmental Sciences, Bioscience Institute,Sao Paulo State University (UNESP), 2Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute,Sao Paulo State University (UNESP)

Chapters

  • 00:04Introduction
  • 00:44Peptide Extraction: Cell Culture
  • 01:30Peptide Extraction: Animal Tissues
  • 03:39Peptide Quantification with Fluorescamine
  • 04:18Reductive Methylation of Amines Labeling
  • 05:58Results: Determining Labelled Peptides Using Mass Spectroscopy
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了一种基于热灭活的样品制备方法，以保存内源性肽，避免死后降解，然后使用同位素标记和LC-MS进行相对定量。

Tags

Sample PreparationRelative QuantitationReductive MethylationAminesPeptidomicsProtease InactivationIsotopic LabelingNeuroblastoma CellsZebrafish BrainCellular LysateUltrafiltrationSolid-phase Extraction

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

RNA 结合区质量谱鉴定的样品制备

用于大分子 X 射线晶体学的一体式样品支架,具有最小的背景散射

使用自动工作站进行质谱测量的等离子体样品制备

探头电喷电镀质谱的样品制备

蛋白质组学设施提交和后续数据分析的TMT样品制备

使用 HPLC-MS/MS 进行核苷/核苷酸含量测量的工厂样品制备

VMXm 光束线微晶样品制备管道

大规模多组学全基因组关联研究 （Mo-GWAS）：样品制备和归一化指南

冷冻电镜高温样品网格的制备

使用脂质单层方法进行电子晶体学研究的样品制备

Read Article