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Extração do Cofactor F420 para Análise do Comprimento da Cauda poliglutama de culturas puras e amostras ambientais
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JoVE Journal Environment
Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

Extração do Cofactor F420 para Análise do Comprimento da Cauda poliglutama de culturas puras e amostras ambientais

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2,434 Views

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04:32 min

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October 14, 2021

DOI:

10.3791/62737-v

DOI:
10.3791/62737-v

•

04:32 min
October 14, 2021

•

7 Views
, , , , ,
1Department of Microbiology,Universität Innsbruck, 2Department of Hygiene and Medical Microbiology,Medical University of Innsbruck

Summary

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Um método para a extração do cofato F420 de culturas puras foi otimizado para a separação e análise cromatográfica líquida do comprimento da cauda F420 em cultura pura e amostras ambientais.

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Markt, R., Wunderer, M., Prem, E. M., Mutschlechner, M., Lackner, N., Wagner, A. O. Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples. J. Vis. Exp. (176), e62737, doi:10.3791/62737 (2021).

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