Extraction of Cofactor F<sub>420</sub> for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

Rudolf Markt; Mathias Wunderer; Eva Maria Prem; Mira Mutschlechner; Nina Lackner; Andreas  Otto Wagner

doi:10.3791/62737

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Extraction of Cofactor F₄₂₀ for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

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Environnement

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Journal JoVE Environnement

Extraction of Cofactor F₄₂₀ for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

2,434 Views

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04:32 min

•

October 14, 2021

DOI:

10.3791/62737-v

DOI:

10.3791/62737-v

•

04:32 min

October 14, 2021

•

7 Views

Rudolf Markt¹, Mathias Wunderer¹, Eva Maria Prem¹, Mira Mutschlechner¹, Nina Lackner², Andreas Otto Wagner¹

¹Department of Microbiology,Universität Innsbruck, ²Department of Hygiene and Medical Microbiology,Medical University of Innsbruck

Summary

A method for the extraction of cofactor F₄₂₀ from pure cultures was optimized for the liquid chromatographic separation and analysis of F₄₂₀ tail length in pure culture and environmental samples.

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Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

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Extraction of Cofactor F₄₂₀ for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples