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Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
 

Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

Article DOI: 10.3791/62737-v • 04:32 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1, 1, 2, 1
1Department of Microbiology, Universität Innsbruck, 2Department of Hygiene and Medical Microbiology, Medical University of Innsbruck

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A method for the extraction of cofactor F420 from pure cultures was optimized for the liquid chromatographic separation and analysis of F420 tail length in pure culture and environmental samples.

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Cofactor F420 Analysis Polyglutamate Tail Length Methanogenic Pure Cultures Environmental Samples Primary Metabolism Secondary Metabolism Prokaryotes Prevalence Function Sample Materials Sludge Soil Lysis Pre-purification Sample Preparation Buffers Conical Tube Lysis Buffer Distilled Water Concentration Vortex Autoclaving Forest Soil Volume Centrifuge Lysate Supernatant Solid-phase Extraction (SPE) Columns
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