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Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
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Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

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04:32 min

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October 14, 2021

DOI:

10.3791/62737-v

DOI:
10.3791/62737-v

•

04:32 min
October 14, 2021

•

7 Views
, , , , ,
1Department of Microbiology,Universität Innsbruck, 2Department of Hygiene and Medical Microbiology,Medical University of Innsbruck

Summary

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A method for the extraction of cofactor F420 from pure cultures was optimized for the liquid chromatographic separation and analysis of F420 tail length in pure culture and environmental samples.

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Cofactor F420MethanogenicPolyglutamateEnvironmental SamplesSample LysisSolid-phase ExtractionHPLC Analysis

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Markt, R., Wunderer, M., Prem, E. M., Mutschlechner, M., Lackner, N., Wagner, A. O. Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples. J. Vis. Exp. (176), e62737, doi:10.3791/62737 (2021).

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