Journal
/
环境科学
/
Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
JoVE 杂志
环境科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 环境科学
Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

DOI:
10.3791/62737-v

•

04:32 min

•

October 14, 2021

•

, , , , ,
1Department of Microbiology,Universität Innsbruck, 2Department of Hygiene and Medical Microbiology,Medical University of Innsbruck

Chapters

  • 00:05Introduction
  • 00:49Sample Lysis and Pre-Purification of the Cofactor F420 via Solid-Phase Extraction (SPE)
  • 02:18Detection of the Cofactor F420
  • 03:02Results: Different F420 Tail-Length Profiles for Methanogens
  • 03:56Conclusion

Summary

自动翻译

自动翻译

A method for the extraction of cofactor F420 from pure cultures was optimized for the liquid chromatographic separation and analysis of F420 tail length in pure culture and environmental samples.

Tags

Keywords: Cofactor F420MethanogenicPolyglutamateEnvironmental SamplesSample LysisSolid-phase ExtractionHPLC Analysis

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/62737?article_type=v

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples
check_url/cn/62737?article_type=v

Use of Chironomidae (Diptera) Surface-Floating Pupal Exuviae as a Rapid Bioassessment Protocol for Water Bodies
check_url/cn/62737?article_type=v

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
check_url/cn/62737?article_type=v

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
check_url/cn/62737?article_type=v

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
check_url/cn/62737?article_type=v

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
check_url/cn/62737?article_type=v

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
check_url/cn/62737?article_type=v

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
check_url/cn/62737?article_type=v

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
check_url/cn/62737?article_type=v

Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis

Read Article