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Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
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JoVE Journal Environment
Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

DOI:
10.3791/62737-v

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04:32 min

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October 14, 2021

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, , , , ,
1Department of Microbiology,Universität Innsbruck, 2Department of Hygiene and Medical Microbiology,Medical University of Innsbruck

Chapters

  • 00:05Introduction
  • 00:49Sample Lysis and Pre-Purification of the Cofactor F420 via Solid-Phase Extraction (SPE)
  • 02:18Detection of the Cofactor F420
  • 03:02Results: Different F420 Tail-Length Profiles for Methanogens
  • 03:56Conclusion

Summary

Automatic Translation

Automatic Translation

A method for the extraction of cofactor F420 from pure cultures was optimized for the liquid chromatographic separation and analysis of F420 tail length in pure culture and environmental samples.

Tags

Keywords: Cofactor F420MethanogenicPolyglutamateEnvironmental SamplesSample LysisSolid-phase ExtractionHPLC Analysis

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