Rivista
/
Ambiente
/
Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
JoVE Journal
Ambiente
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Ambiente
Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

DOI:
10.3791/62737-v

•

04:32 min

•

October 14, 2021

•

, , , , ,
1Department of Microbiology,Universität Innsbruck, 2Department of Hygiene and Medical Microbiology,Medical University of Innsbruck

Capitoli

  • 00:05Introduction
  • 00:49Sample Lysis and Pre-Purification of the Cofactor F420 via Solid-Phase Extraction (SPE)
  • 02:18Detection of the Cofactor F420
  • 03:02Results: Different F420 Tail-Length Profiles for Methanogens
  • 03:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

A method for the extraction of cofactor F420 from pure cultures was optimized for the liquid chromatographic separation and analysis of F420 tail length in pure culture and environmental samples.

Tags

Cofactor F420MethanogenicPolyglutamateEnvironmental SamplesSample LysisSolid-phase ExtractionHPLC Analysis

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples

Use of Chironomidae (Diptera) Surface-Floating Pupal Exuviae as a Rapid Bioassessment Protocol for Water Bodies

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis

Read Article