A method for the extraction of cofactor F420 from pure cultures was optimized for the liquid chromatographic separation and analysis of F420 tail length in pure culture and environmental samples.
Markt, R., Wunderer, M., Prem, E. M., Mutschlechner, M., Lackner, N., Wagner, A. O. Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples. J. Vis. Exp. (176), e62737, doi:10.3791/62737 (2021).