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A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
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A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

English

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19,130 Views

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17:39 min

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July 16, 2017

DOI:

10.3791/55310-v

DOI:
10.3791/55310-v

•

17:39 min
July 16, 2017

•

5 Views
, , , , ,
1Department of Agronomy and Great Lakes Bioenergy Research Center,University of Wisconsin – Madison, 2Department of Soil Science,University of Wisconsin – Madison, 3Department of Soil, Water, and Climate,University of Minnesota, 4Faculty of Science and Engineering,Curtin University

Summary

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The article describes a method that increases throughput while balancing effort and accuracy for extraction of lipids from the cell membranes of microorganisms for use in characterizing both total lipids and the relative abundance of indicator lipids to determine soil microbial community structure in studies with many samples.

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Cite this Article

Oates, L. G., Read, H. W., Gutknecht, J. L. M., Duncan, D. S., Balser, T. B., Jackson, R. D. A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples. J. Vis. Exp. (125), e55310, doi:10.3791/55310 (2017).

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