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Ganglioside Extraction, Purification and Profiling
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Biochemistry
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JoVE Journal Biochemistry
Ganglioside Extraction, Purification and Profiling
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Ganglioside Extraction, Purification and Profiling

DOI:
10.3791/62385-v

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10:05 min

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March 12, 2021

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, ,
1Department of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, 2Department of Neuroscience,Johns Hopkins University School of Medicine

Chapters

  • 00:04Introduction
  • 01:36Small Scale Ganglioside Extraction and Partial Purification
  • 03:35Large Scale Ganglioside Extraction and Purification
  • 05:52Ganglioside Resolution and Staining
  • 08:02Results: Small and Large Scale Ganglioside Extraction and Partial Purification
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

Gangliosides are sialic acid-bearing glycosphingolipids that are particularly abundant in the brain. Their amphipathic nature requires organic/aqueous extraction and purification techniques to ensure optimal recovery and accurate analyses. This article provides overviews of analytic and preparative scale ganglioside extraction, purification, and thin layer chromatography analysis.

Tags

Ganglioside ExtractionGanglioside PurificationGanglioside ProfilingCell Surface LipidsNerve Cell LipidsCell SignalingCell-cell RecognitionPolar Lipid AnalysisReverse-phase Cartridge ChromatographySolvent ExtractionPartitioningSolid-phase Extraction

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