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Ganglioside Extraction, Purification and Profiling
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JoVE Journal Biochimica
Ganglioside Extraction, Purification and Profiling

Ganglioside Extraction, Purification and Profiling

4,025 Views

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10:05 min

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March 12, 2021

DOI:

10.3791/62385-v

DOI:
10.3791/62385-v

•

10:05 min
March 12, 2021

•

28 Views
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1Department of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, 2Department of Neuroscience,Johns Hopkins University School of Medicine

Summary

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Gangliosides are sialic acid-bearing glycosphingolipids that are particularly abundant in the brain. Their amphipathic nature requires organic/aqueous extraction and purification techniques to ensure optimal recovery and accurate analyses. This article provides overviews of analytic and preparative scale ganglioside extraction, purification, and thin layer chromatography analysis.

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Cite this Article

Porter, M. J., Zhang, G., Schnaar, R. L. Ganglioside Extraction, Purification and Profiling. J. Vis. Exp. (169), e62385, doi:10.3791/62385 (2021).

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