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Expression, Purification, and Antimicrobial Activity of S100A12
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Biochemistry
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JoVE Journal Biochemistry
Expression, Purification, and Antimicrobial Activity of S100A12

Expression, Purification, and Antimicrobial Activity of S100A12

English

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8,197 Views

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11:10 min

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May 13, 2017

DOI:

10.3791/55557-v

DOI:
10.3791/55557-v

•

11:10 min
May 13, 2017

•

9 Views
, , , ,
1Department of Life and Physical Sciences,Fisk University, 2Tennessee Valley Healthcare Systems,U. S. Dept. of Veterans Affairs, 3Department of Medicine – Division of Infectious Diseases,Vanderbilt University Medical School, 4Department of Biochemistry,Vanderbilt University

Summary

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Here, we present a method to express and purify S100A12 (calgranulin C). We describe a protocol to measure its antimicrobial activity against the human pathogen H. pylori.

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Jackson, E., Little, S., Franklin, D. S., Gaddy, J. A., Damo, S. M. Expression, Purification, and Antimicrobial Activity of S100A12. J. Vis. Exp. (123), e55557, doi:10.3791/55557 (2017).

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