Journal
/
Bioquímica
/
Ganglioside Extraction, Purification and Profiling
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Ganglioside Extraction, Purification and Profiling

Ganglioside Extraction, Purification and Profiling

4,025 Views

•

10:05 min

•

March 12, 2021

DOI:

10.3791/62385-v

DOI:
10.3791/62385-v

•

10:05 min
March 12, 2021

•

28 Views
, ,
1Department of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, 2Department of Neuroscience,Johns Hopkins University School of Medicine

Summary

Automatically generated

Gangliosides are sialic acid-bearing glycosphingolipids that are particularly abundant in the brain. Their amphipathic nature requires organic/aqueous extraction and purification techniques to ensure optimal recovery and accurate analyses. This article provides overviews of analytic and preparative scale ganglioside extraction, purification, and thin layer chromatography analysis.

Explore More Videos

Ganglioside ExtractionGanglioside PurificationGanglioside ProfilingCell Surface LipidsNerve Cell LipidsCell SignalingCell-cell RecognitionPolar Lipid AnalysisReverse-phase Cartridge ChromatographySolvent ExtractionPartitioningSolid-phase Extraction

Read Article

Cite this Article

Porter, M. J., Zhang, G., Schnaar, R. L. Ganglioside Extraction, Purification and Profiling. J. Vis. Exp. (169), e62385, doi:10.3791/62385 (2021).

Download .ris file

Copy

Share Video

.

Copy