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Ganglioside extractie, zuivering en profilering
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Biochemistry
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JoVE Journal Biochemistry
Ganglioside Extraction, Purification and Profiling
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Ganglioside extractie, zuivering en profilering

Nederlands

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4,027 Views

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10:05 min

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March 12, 2021

DOI:

10.3791/62385-v

DOI:
10.3791/62385-v

•

10:05 min
March 12, 2021

•

28 Views
, ,
1Department of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, 2Department of Neuroscience,Johns Hopkins University School of Medicine

Summary

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Gangliosiden zijn siaalzuurhoudende glycosfingolipiden die bijzonder overvloedig aanwezig zijn in de hersenen. Hun amfipathische aard vereist organische /waterige extractie- en zuiveringstechnieken om optimaal herstel en nauwkeurige analyses te garanderen. Dit artikel biedt een overzicht van analytische en preparatieve schaal ganglioside extractie, zuivering en dunne laag chromatografie analyse.

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Cite this Article

Porter, M. J., Zhang, G., Schnaar, R. L. Ganglioside Extraction, Purification and Profiling. J. Vis. Exp. (169), e62385, doi:10.3791/62385 (2021).

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