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Polysome Profiling in Leishmania, Human Cells and Mouse Testis
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Biochemistry
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JoVE Journal Biochemistry
Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

17,248 Views

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14:32 min

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April 08, 2018

DOI:

10.3791/57600-v

DOI:
10.3791/57600-v

•

14:32 min
April 08, 2018

•

6 Views
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1Department of Cell Biology and Biochemistry,Texas Tech University Health Sciences Center, 2Department of Biological Sciences,Texas Tech University, 3CISER (Center for the Integration of STEM Education & Research),Texas Tech University

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Polysome ProfilingMRNA TranslationProtein Synthesis RegulationLeishmaniaHuman CellsMouse TestisSucrose GradientUltracentrifugationRibosome FractionationCytoplasmic LysateCycloheximideCell CulturePBS WashCell Counting

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Karamysheva, Z. N., Tikhonova, E. B., Grozdanov, P. N., Huffman, J. C., Baca, K. R., Karamyshev, A., Denison, R. B., MacDonald, C. C., Zhang, K., Karamyshev, A. L. Polysome Profiling in Leishmania, Human Cells and Mouse Testis. J. Vis. Exp. (134), e57600, doi:10.3791/57600 (2018).

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