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Polysome Profiling in Leishmania, Human Cells and Mouse Testis
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Biochemistry
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JoVE Journal Biochemistry
Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

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17,347 Views

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14:32 min

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April 08, 2018

DOI:

10.3791/57600-v

DOI:
10.3791/57600-v

•

14:32 min
April 08, 2018

•

9 Views
, , , , , , , , ,
1Department of Cell Biology and Biochemistry,Texas Tech University Health Sciences Center, 2Department of Biological Sciences,Texas Tech University, 3CISER (Center for the Integration of STEM Education & Research),Texas Tech University

Summary

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The overall goal of polysome profiling technique is analysis of translational activity of individual mRNAs or transcriptome mRNAs during protein synthesis. The method is important for studies of protein synthesis regulation, translation activation and repression in health and multiple human diseases.

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Polysome ProfilingMRNA TranslationProtein Synthesis RegulationLeishmaniaHuman CellsMouse TestisSucrose GradientUltracentrifugationRibosome FractionationCytoplasmic LysateCycloheximideCell CulturePBS WashCell Counting

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Karamysheva, Z. N., Tikhonova, E. B., Grozdanov, P. N., Huffman, J. C., Baca, K. R., Karamyshev, A., Denison, R. B., MacDonald, C. C., Zhang, K., Karamyshev, A. L. Polysome Profiling in Leishmania, Human Cells and Mouse Testis. J. Vis. Exp. (134), e57600, doi:10.3791/57600 (2018).

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