A subscription to JoVE is required to view this content. Sign in or start your free trial.
16,939 Views
•
09:54 min
•
January 24, 2017
DOI:
Summary
Automatically generated
This protocol describes the production of a therapeutic antibody in a mammalian expression system. The methods described include preparation of vector DNA, stable transfection and serum-free adaptation of a human embryonic kidney 293 cell line, set up of large scale cultures and purification using affinity chromatography.
Read Article
Cite this Article
Elgundi, Z., Sifniotis, V., Reslan, M., Cruz, E., Kayser, V. Laboratory Scale Production and Purification of a Therapeutic Antibody. J. Vis. Exp. (119), e55153, doi:10.3791/55153 (2017).
Copy