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Isolierung von Histon von Sorghum Blattgewebe für Top Down Mass Spektrometrie Profilierung von potenziellen epigenetischen Markern
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Biochemistry
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JoVE Journal Biochemistry
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
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Isolierung von Histon von Sorghum Blattgewebe für Top Down Mass Spektrometrie Profilierung von potenziellen epigenetischen Markern

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4,428 Views

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07:10 min

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March 04, 2021

DOI:

10.3791/61707-v

DOI:
10.3791/61707-v

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07:10 min
March 04, 2021

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2 Views
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1Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute,Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center,University of California Agriculture and Natural Resources, 4West Side Research and Extension Center,University of California, 5Department of Plant Sciences,University of California, Davis, 6Department of Plant and Microbial Biology,University of California, Berkeley

Summary

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Das Protokoll wurde entwickelt, um intakte Histone effektiv aus Sorghumblattmaterialien für die Profilierung von histonenposttranslationalen Modifikationen zu extrahieren, die als potenzielle epigenetische Marker dienen können, um die Konstruktion von dürreresistenten Kulturen zu unterstützen.

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Histone ExtractionSorghumMass SpectrometryEpigenetic MarkersPost-translational ModificationsProtease InhibitorsNuclei Lysis BufferCentrifugation

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Cite this Article

Zhou, M., Abdali, S. H., Dilworth, D., Liu, L., Cole, B., Malhan, N., Ahkami, A. H., Winkler, T. E., Hollingsworth, J., Sievert, J., Dahlberg, J., Hutmacher, R., Madera, M., Owiti, J. A., Hixson, K. K., Lemaux, P. G., Jansson, C., Paša-Tolić, L. Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers. J. Vis. Exp. (169), e61707, doi:10.3791/61707 (2021).

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