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Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
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Biochemistry
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JoVE Journal Biochemistry
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

DOI:
10.3791/61707-v

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07:10 min

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March 04, 2021

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, , , , , , , , , , , , , , , , ,
1Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute,Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center,University of California Agriculture and Natural Resources, 4West Side Research and Extension Center,University of California, 5Department of Plant Sciences,University of California, Davis, 6Department of Plant and Microbial Biology,University of California, Berkeley

Chapters

  • 00:05Introduction
  • 00:40Nuclei Isolation from Sorghum Leaf Sample
  • 02:13Isolation of Nuclei from Remaining Cytoplasmic Contaminants
  • 03:05Nuclei Lysis and Histone Extraction
  • 04:39Results: Qualitative & Quantitative Analysis of the Identified Histones
  • 06:36Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol has been developed to effectively extract intact histones from sorghum leaf materials for profiling of histone post-translational modifications that can serve as potential epigenetic markers to aid engineering drought resistant crops.

Tags

Histone ExtractionSorghumMass SpectrometryEpigenetic MarkersPost-translational ModificationsProtease InhibitorsNuclei Lysis BufferCentrifugation

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