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Isolatie van histone van Sorghum Leaf Tissue voor Top Down Mass Spectrometry Profiling van potentiële epigenetische markers
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March 04, 2021
DOI:
1Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute,Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center,University of California Agriculture and Natural Resources, 4West Side Research and Extension Center,University of California, 5Department of Plant Sciences,University of California, Davis, 6Department of Plant and Microbial Biology,University of California, Berkeley
Summary
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Het protocol is ontwikkeld om intacte histonen effectief te extraheren uit sorghumbladmaterialen voor profilering van histon post-translationele modificaties die kunnen dienen als potentiële epigenetische markers om droogtebestendige gewassen te helpen.
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Zhou, M., Abdali, S. H., Dilworth, D., Liu, L., Cole, B., Malhan, N., Ahkami, A. H., Winkler, T. E., Hollingsworth, J., Sievert, J., Dahlberg, J., Hutmacher, R., Madera, M., Owiti, J. A., Hixson, K. K., Lemaux, P. G., Jansson, C., Paša-Tolić, L. Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers. J. Vis. Exp. (169), e61707, doi:10.3791/61707 (2021).
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