Journal
/
Biochemistry
/
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

English

Automatically Generated

11,716 Views

•

10:37 min

•

November 15, 2017

DOI:

10.3791/56474-v

DOI:
10.3791/56474-v

•

10:37 min
November 15, 2017

•

7 Views
, , , , , , ,
1St. Jude Proteomics Facility,St. Jude Children’s Research Hospital, 2Departments of Structural Biology and Developmental Neurobiology,St. Jude Children’s Research Hospital, 3Heilongjiang University of Chinese Medicine

Summary

Automatically generated

We present a protocol to accurately quantitate proteins with isobaric labelling, extensive fractionation, bioinformatics tools, and quality control steps in combination with liquid chromatography interfaced to a high-resolution mass spectrometer.

Explore More Videos

Proteome ProfilingIsobaric LabelingLiquid ChromatographyMass SpectrometryQuantificationProtein IdentificationProtein QuantitationBiomarker DiscoveryCell LysisProtein DigestionAcetonitrileLys-C ProteaseDTTHEPES

Read Article

Cite this Article

High, A. A., Tan, H., Pagala, V. R., Niu, M., Cho, J., Wang, X., Bai, B., Peng, J. Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification. J. Vis. Exp. (129), e56474, doi:10.3791/56474 (2017).

Download .ris file

Copy

Share Video

.

Copy