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Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
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Biochemistry
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JoVE Journal Biochemistry
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

English

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9,829 Views

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10:23 min

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April 17, 2017

DOI:

10.3791/55585-v

DOI:
10.3791/55585-v

•

10:23 min
April 17, 2017

•

16 Views
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1Heinrich Pette Institute,Leibniz Institute for Experimental Virology, 2Core Facility Mass Spectrometric Proteomics,University Medical Center Hamburg-Eppendorf

Summary

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Lipid droplets are important organelles for the replication of several pathogens, including the Hepatitis C Virus (HCV). We describe a method to isolate lipid droplets for quantitative mass spectrometry of associated proteins; it can be used under a variety of conditions, such as virus infection, environmental stress, or drug treatment.

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Cite this Article

Rösch, K., Kwiatkowski, M., Schlüter, H., Herker, E. Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis. J. Vis. Exp. (122), e55585, doi:10.3791/55585 (2017).

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