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Isolamento de Histone do tecido da folha de sorgo para perfil de espectrometria de massa de top down de potenciais marcadores epigenéticos
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Biochemistry
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JoVE Journal Biochemistry
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Isolamento de Histone do tecido da folha de sorgo para perfil de espectrometria de massa de top down de potenciais marcadores epigenéticos

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4,428 Views

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07:10 min

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March 04, 2021

DOI:

10.3791/61707-v

DOI:
10.3791/61707-v

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07:10 min
March 04, 2021

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1Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute,Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center,University of California Agriculture and Natural Resources, 4West Side Research and Extension Center,University of California, 5Department of Plant Sciences,University of California, Davis, 6Department of Plant and Microbial Biology,University of California, Berkeley

Summary

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O protocolo foi desenvolvido para extrair efetivamente histonas intactas de materiais de folhas de sorgo para o perfil de modificações pós-translacionais de histona que podem servir como potenciais marcadores epigenéticos para ajudar as culturas resistentes à seca de engenharia.

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Histone ExtractionSorghumMass SpectrometryEpigenetic MarkersPost-translational ModificationsProtease InhibitorsNuclei Lysis BufferCentrifugation

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Cite this Article

Zhou, M., Abdali, S. H., Dilworth, D., Liu, L., Cole, B., Malhan, N., Ahkami, A. H., Winkler, T. E., Hollingsworth, J., Sievert, J., Dahlberg, J., Hutmacher, R., Madera, M., Owiti, J. A., Hixson, K. K., Lemaux, P. G., Jansson, C., Paša-Tolić, L. Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers. J. Vis. Exp. (169), e61707, doi:10.3791/61707 (2021).

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