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Diş Hamuru Parakrin Sinyalleri yanıt olarak Neurite Outgrowth Çalışma Co-Kültür Yöntemi
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Developmental Biology
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JoVE Journal Developmental Biology
A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
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Diş Hamuru Parakrin Sinyalleri yanıt olarak Neurite Outgrowth Çalışma Co-Kültür Yöntemi

Türkçe

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5,871 Views

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08:06 min

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February 14, 2020

DOI:

10.3791/60809-v

DOI:
10.3791/60809-v

•

08:06 min
February 14, 2020

•

1 Views
, ,
1Cell, Developmental and Integrative Biology Department,University of Alabama at Birmingham

Summary

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Biz izolasyon, dağılım ve diş hamuru kaplama (DP) trigeminal ile birincil hücrelerin kaplama (TG) nöronlar üstteki transwell filtreleri üzerinde kültürlü. DP hücrelerinin hücresel yanıtları immünofloresan veya RNA/protein analizi ile analiz edilebilir. Konfokal mikroskopi ile nöronal belirteçlerin immünfloresansi neurite outgrowth yanıtlarının analizine izin verir.

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Cite this Article

Barkley, C., Serra, R., Peters, S. B. A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals. J. Vis. Exp. (156), e60809, doi:10.3791/60809 (2020).

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