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研究对牙髓寄生虫信号反应的神经质生长的共同培养方法
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Developmental Biology
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JoVE Journal Developmental Biology
A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
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研究对牙髓寄生虫信号反应的神经质生长的共同培养方法

English

Automatically Generated

5,863 Views

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08:06 min

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February 14, 2020

DOI:

10.3791/60809-v

DOI:
10.3791/60809-v

•

08:06 min
February 14, 2020

•

1 Views
, ,
1Cell, Developmental and Integrative Biology Department,University of Alabama at Birmingham

Summary

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我们描述了牙髓（DP）原细胞的分离、分散和电镀，这些细胞在覆盖的透井过滤器上培养三叉（TG）神经元。DP细胞的细胞反应可以通过免疫荧光或RNA/蛋白质分析进行分析。神经元标记物与共聚焦显微镜的免疫荧光允许分析神经质外生长反应。

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Cite this Article

Barkley, C., Serra, R., Peters, S. B. A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals. J. Vis. Exp. (156), e60809, doi:10.3791/60809 (2020).

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