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Een co-cultuur methode om Neurite Outgrowth studie in reactie op tandheelkundige pulp paracrine signalen
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Developmental Biology
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JoVE Journal Developmental Biology
A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
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Een co-cultuur methode om Neurite Outgrowth studie in reactie op tandheelkundige pulp paracrine signalen

Nederlands

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5,870 Views

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08:06 min

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February 14, 2020

DOI:

10.3791/60809-v

DOI:
10.3791/60809-v

•

08:06 min
February 14, 2020

•

1 Views
, ,
1Cell, Developmental and Integrative Biology Department,University of Alabama at Birmingham

Summary

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We beschrijven de isolatie, dispersie en beplating van tandheelkundige pulp (DP) primaire cellen met trigeminale (TG) neuronen gekweekt bovenop bovenliggende transwell filters. Cellulaire reacties van DP-cellen kunnen worden geanalyseerd met immunofluorescentie of RNA/eiwitanalyse. Immunofluorescentie van neuronale markers met confocale microscopie maakt de analyse van neurite outgrowth reacties mogelijk.

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Cite this Article

Barkley, C., Serra, R., Peters, S. B. A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals. J. Vis. Exp. (156), e60809, doi:10.3791/60809 (2020).

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