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A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals

A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals

DOI:
10.3791/60809-v

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08:06 min

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February 14, 2020

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, ,
1Cell, Developmental and Integrative Biology Department,University of Alabama at Birmingham

Capitoli

  • 00:04Introduction
  • 00:58DP (Dental Pulp) Dissection, Dispersion and Plating
  • 03:24Trigeminal Neuron Dissection, Dispersion and Plating
  • 06:01Results: Co-culture
  • 07:20Conclusion

Summary

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We describe the isolation, dispersion and plating of dental pulp (DP) primary cells with trigeminal (TG) neurons cultured atop overlying transwell filters. Cellular responses of DP cells can be analyzed with immunofluorescence or RNA/protein analysis. Immunofluorescence of neuronal markers with confocal microscopy permits the analysis of neurite outgrowth responses.

Tags

Co-cultureNeurite OutgrowthDental PulpParacrine SignalsTrigeminal NeuronsNeural ResearchMouse DissectionDental Pulp Stem CellsTrypsinization

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