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Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

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7,154 Views

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11:50 min

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March 18, 2019

DOI:

10.3791/59282-v

DOI:
10.3791/59282-v

•

11:50 min
March 18, 2019

•

9 Views
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1Laboratory of Experimental Medicine and Environmental Pathology,Sabina Universitas – Rieti University Hub, 2Department of Experimental Medicine,Sapienza University of Rome, 3Department of Science Dentistry and Maxillofacial,Sapienza University of Rome

Summary

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Here we present a protocol for human Dental Pulp Stem Cells isolation and propagation in order to evaluate the prion protein expression during neuronal differentiation process.

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Dental Pulp Stem CellsIsolationPropagationPrion Protein ExpressionNeuronal Differentiation

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Martellucci, S., Santacroce, C., Manganelli, V., Santilli, F., Piccoli, L., Cassetta, M., Misasi, R., Sorice, M., Mattei, V. Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells. J. Vis. Exp. (145), e59282, doi:10.3791/59282 (2019).

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