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Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

DOI:
10.3791/59282-v

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11:50 min

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March 18, 2019

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, , , , , , , ,
1Laboratory of Experimental Medicine and Environmental Pathology,Sabina Universitas – Rieti University Hub, 2Department of Experimental Medicine,Sapienza University of Rome, 3Department of Science Dentistry and Maxillofacial,Sapienza University of Rome

Chapters

  • 00:04Title
  • 00:47Tooth and Dental Pulp Extraction
  • 01:29Processing of the Dental Pulp and Stem Cell Release
  • 02:29Stem Cell Culture and Propagation
  • 03:44Transient PrPC Silencing by siRNA
  • 05:04Neuronal Induction Process of hDPSCs
  • 05:50Characterization of hDPSCs by Flow Cytometry
  • 08:03Evaluation of PrPC Expression in hDPSCs by Flow Cytometry Analysis
  • 09:14Results: hDPSCs Isolation and Propagation and the Role of PrP<supC During Neuronal Differentiation
  • 10:54Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol for human Dental Pulp Stem Cells isolation and propagation in order to evaluate the prion protein expression during neuronal differentiation process.

Tags

Dental Pulp Stem CellsIsolationPropagationPrion Protein ExpressionNeuronal Differentiation

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