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A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
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A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
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February 14, 2020
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We describe the isolation, dispersion and plating of dental pulp (DP) primary cells with trigeminal (TG) neurons cultured atop overlying transwell filters. Cellular responses of DP cells can be analyzed with immunofluorescence or RNA/protein analysis. Immunofluorescence of neuronal markers with confocal microscopy permits the analysis of neurite outgrowth responses.
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Barkley, C., Serra, R., Peters, S. B. A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals. J. Vis. Exp. (156), e60809, doi:10.3791/60809 (2020).
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