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Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

English

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5,979 Views

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12:27 min

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January 03, 2020

DOI:

10.3791/60681-v

DOI:
10.3791/60681-v

•

12:27 min
January 03, 2020

•

10 Views
, , , , , , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Summary

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This protocol describes a method for the purification of polymorphonuclear leukocytes from whole human blood and two distinct assays that quantify the cytotoxicity of Staphylococcus aureus against these important innate immune cells.

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Staphylococcus AureusCytotoxicityPolymorphonuclear LeukocytesPMNsNeutrophilsBi-component LeukotoxinsMembrane IntegrityInnate Immune CellsCentrifugationDensity GradientsHeparinized Whole BloodDextranFicollCell IsolationCell Separation

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Dankoff, J. G., Pallister, K. B., Guerra, F. E., Parks, A. J., Gorham, K., Mastandrea, S., Voyich, J. M., Nygaard, T. K. Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes. J. Vis. Exp. (155), e60681, doi:10.3791/60681 (2020).

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