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Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
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Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
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January 03, 2020
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This protocol describes a method for the purification of polymorphonuclear leukocytes from whole human blood and two distinct assays that quantify the cytotoxicity of Staphylococcus aureus against these important innate immune cells.
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Dankoff, J. G., Pallister, K. B., Guerra, F. E., Parks, A. J., Gorham, K., Mastandrea, S., Voyich, J. M., Nygaard, T. K. Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes. J. Vis. Exp. (155), e60681, doi:10.3791/60681 (2020).
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