ジャーナル
/
免疫学と感染
/
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
JoVE Journal
免疫学と感染
Author Produced
This content is Free Access.
JoVE Journal 免疫学と感染
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

5,979 Views

•

12:27 min

•

January 03, 2020

DOI:

10.3791/60681-v

DOI:
10.3791/60681-v

•

12:27 min
January 03, 2020

•

10 Views
, , , , , , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

概要

Automatically generated

This protocol describes a method for the purification of polymorphonuclear leukocytes from whole human blood and two distinct assays that quantify the cytotoxicity of Staphylococcus aureus against these important innate immune cells.

Explore More Videos

Staphylococcus AureusCytotoxicityPolymorphonuclear LeukocytesPMNsNeutrophilsBi-component LeukotoxinsMembrane IntegrityInnate Immune CellsCentrifugationDensity GradientsHeparinized Whole BloodDextranFicollCell IsolationCell Separation

Read Article

Cite this Article

Dankoff, J. G., Pallister, K. B., Guerra, F. E., Parks, A. J., Gorham, K., Mastandrea, S., Voyich, J. M., Nygaard, T. K. Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes. J. Vis. Exp. (155), e60681, doi:10.3791/60681 (2020).

Download .ris file

Copy

Share Video

.

Copy