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Quantifizierung der Zytotoxizität von Staphyloccus aureus gegen humane polymorphonukleare Leukozyten
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
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Quantifizierung der Zytotoxizität von Staphyloccus aureus gegen humane polymorphonukleare Leukozyten

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5,980 Views

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12:27 min

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January 03, 2020

DOI:

10.3791/60681-v

DOI:
10.3791/60681-v

•

12:27 min
January 03, 2020

•

10 Views
, , , , , , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Summary

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Dieses Protokoll beschreibt eine Methode zur Reinigung polymorphonuklearer Leukozyten aus ganzem menschlichen Blut und zwei verschiedene Assays, die die Zytotoxizität von Staphylococcus aureus gegen diese wichtigen angeborenen Immunzellen quantifizieren.

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Dankoff, J. G., Pallister, K. B., Guerra, F. E., Parks, A. J., Gorham, K., Mastandrea, S., Voyich, J. M., Nygaard, T. K. Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes. J. Vis. Exp. (155), e60681, doi:10.3791/60681 (2020).

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