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Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets

Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets

English

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36,654 Views

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11:40 min

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March 27, 2014

DOI:

10.3791/51301-v

DOI:
10.3791/51301-v

•

11:40 min
March 27, 2014

•

7 Views
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1UAB Mitochondrial Medicine Laboratory, Center for Free Radical Biology, Department of Pathology,University of Alabama at Birmingham

Summary

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Blood leukocytes and platelets can be used as a marker of overall bioenergetic health of an individual and so have the potential to monitor pathological processes and the impact of treatments. Here we describe a method to isolate and measure mitochondrial function and the oxidative burst in these cells.

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BioenergeticsOxidative BurstLeukocytesPlateletsMitochondrial DysfunctionAtherosclerosisDiabetesSeptic ShockNeurodegenerative DiseasesHyperglycemiaInflammationMagnetic Bead IsolationCell AdhesionMicroplate RespirometryExtracellular Flux AnalyzerMonocytesLymphocytesNeutrophilsReactive Oxygen Species

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Kramer, P. A., Chacko, B. K., Ravi, S., Johnson, M. S., Mitchell, T., Darley-Usmar, V. M. Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets. J. Vis. Exp. (85), e51301, doi:10.3791/51301 (2014).

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