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Quantificare la Citotossicità dello Staphyloccus aureus contro il Polimorfo Umano Leukociti
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
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Quantificare la Citotossicità dello Staphyloccus aureus contro il Polimorfo Umano Leukociti

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5,980 Views

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12:27 min

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January 03, 2020

DOI:

10.3791/60681-v

DOI:
10.3791/60681-v

•

12:27 min
January 03, 2020

•

10 Views
, , , , , , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Summary

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Questo protocollo descrive un metodo per la purificazione dei leucociti polimorfonucleari da tutto il sangue umano e due saggi distinti che quantificano la citotossicità dello Staphylococcus aureus contro queste importanti cellule immunitarie innate.

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Cite this Article

Dankoff, J. G., Pallister, K. B., Guerra, F. E., Parks, A. J., Gorham, K., Mastandrea, S., Voyich, J. M., Nygaard, T. K. Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes. J. Vis. Exp. (155), e60681, doi:10.3791/60681 (2020).

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