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Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

DOI:
10.3791/60681-v

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12:27 min

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January 03, 2020

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, , , , , , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Chapters

  • 00:00Title
  • 00:44Polymorphonuclear Leukocyte Purification
  • 04:34Cytotoxicity Assay of S. aureus Extracellular Proteins Against Human Polymorphonuclear Leukocytes
  • 06:16S. aureus Cytotoxicity Against Human Polymorhonuclear Leukocytes Following Phagocytosis
  • 08:40Results
  • 11:02Conclusion

Summary

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This protocol describes a method for the purification of polymorphonuclear leukocytes from whole human blood and two distinct assays that quantify the cytotoxicity of Staphylococcus aureus against these important innate immune cells.

Tags

Staphylococcus AureusCytotoxicityPolymorphonuclear LeukocytesPMNsNeutrophilsBi-component LeukotoxinsMembrane IntegrityInnate Immune CellsCentrifugationDensity GradientsHeparinized Whole BloodDextranFicollCell IsolationCell Separation

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