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Journal
/
Genetics
/
番茄根转化后与
拉子托尼亚索拉纳卡鲁姆
接种,直接遗传分析细菌性威尔特病
JoVE Journal
Genetics
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JoVE Journal
Genetics
Tomato Root Transformation Followed by Inoculation with
Ralstonia Solanacearum
for Straightforward Genetic Analysis of Bacterial Wilt Disease
Please note that all translations are automatically generated.
Click here for the English version.
番茄根转化后与
拉子托尼亚索拉纳卡鲁姆
接种,直接遗传分析细菌性威尔特病
DOI:
10.3791/60302-v
•
09:05 min
•
March 11, 2020
•
Rafael J. L. Morcillo
,
Achen Zhao
2
,
María I. Tamayo-Navarrete
,
José M. García-Garrido
,
Alberto P. Macho
1
Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences
,
Chinese Academy of Sciences
,
2
University of Chinese Academy of Sciences
,
3
Department of Soil Microbiology and Symbiotic Systems
,
Estación Experimental del Zaidín (CSIC)
Chapters
00:04
Introduction
01:03
Preparation of Tomato Plants and
Agrobacterium Rhizogenes
01:55
Plant Transformation and Selection
04:01
Transfer to Inoculation Pots
04:40
Soil-Drenching Inoculation
05:41
Results: Genetic Analysis of Bacterial Wilt Disease
08:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一种用于番茄根转化的通用方法,然后用
Ralstonia索兰卡鲁姆
接种,对细菌枯萎病的研究进行直接的基因分析。
Tags
Tomato
Root Transformation
Ralstonia Solanacearum
Bacterial Wilt
Genetic Analysis
Agrobacterium Rhizogenes
In Vitro
Sterility
Seedling Manipulation
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