Journal
/
Génétique
/
Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Journal JoVE
Génétique
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Génétique
Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

DOI:
10.3791/60302-v

•

09:05 min

•

March 11, 2020

•

, , , ,
1Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences, 2University of Chinese Academy of Sciences, 3Department of Soil Microbiology and Symbiotic Systems,Estación Experimental del Zaidín (CSIC)

Chapitres

  • 00:04Introduction
  • 01:03Preparation of Tomato Plants and Agrobacterium Rhizogenes
  • 01:55Plant Transformation and Selection
  • 04:01Transfer to Inoculation Pots
  • 04:40Soil-Drenching Inoculation
  • 05:41Results: Genetic Analysis of Bacterial Wilt Disease
  • 08:20Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a versatile method for tomato root transformation followed by inoculation with Ralstonia solanacearum to perform straightforward genetic analysis for the study of bacterial wilt disease.

Tags

TomatoRoot TransformationRalstonia SolanacearumBacterial WiltGenetic AnalysisAgrobacterium RhizogenesIn VitroSterilitySeedling Manipulation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Read Article