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Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
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Genetics
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JoVE Journal Genetics
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

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10,117 Views

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09:26 min

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July 10, 2019

DOI:

10.3791/59913-v

DOI:
10.3791/59913-v

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09:26 min
July 10, 2019

•

11 Views
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1Department of Molecular Genetics, Center for RNA Biology,The Ohio State University

Summary

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Here, we present a protocol to enrich endogenous RNA binding sites or "footprints" of RNA:protein (RNP) complexes from mammalian cells. This approach involves two immunoprecipitations of RNP subunits and is therefore dubbed RNA immunoprecipitation in tandem (RIPiT).

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Woodward, L., Gangras, P., Singh, G. Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq). J. Vis. Exp. (149), e59913, doi:10.3791/59913 (2019).

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