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Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
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Genetica
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JoVE Journal Genetica
Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

DOI:
10.3791/60302-v

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09:05 min

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March 11, 2020

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, , , ,
1Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences, 2University of Chinese Academy of Sciences, 3Department of Soil Microbiology and Symbiotic Systems,Estación Experimental del Zaidín (CSIC)

Capitoli

  • 00:04Introduction
  • 01:03Preparation of Tomato Plants and Agrobacterium Rhizogenes
  • 01:55Plant Transformation and Selection
  • 04:01Transfer to Inoculation Pots
  • 04:40Soil-Drenching Inoculation
  • 05:41Results: Genetic Analysis of Bacterial Wilt Disease
  • 08:20Conclusion

Summary

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Here, we present a versatile method for tomato root transformation followed by inoculation with Ralstonia solanacearum to perform straightforward genetic analysis for the study of bacterial wilt disease.

Tags

TomatoRoot TransformationRalstonia SolanacearumBacterial WiltGenetic AnalysisAgrobacterium RhizogenesIn VitroSterilitySeedling Manipulation

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