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JoVE Journal Genetics
Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
 

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Article DOI: 10.3791/60302-v • 09:05 min • March 11th, 2020
March 11th, 2020 •
1, 1,2, 3, 3, 1
1Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, 2University of Chinese Academy of Sciences, 3Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (CSIC)

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Here, we present a versatile method for tomato root transformation followed by inoculation with Ralstonia solanacearum to perform straightforward genetic analysis for the study of bacterial wilt disease.

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Tomato Root Transformation Inoculation Ralstonia Solanacearum Genetic Analysis Bacterial Wilt Disease Technique Gene Expression Assays Equipment Requirements Plant Growth Space Versatility Crop Plants Pathogen Inoculation Physiological Assays Sterility Maintenance Seedlings Manipulation Visualization Implementation Achen Zhao Graduate Student Sterilization Tomato Seeds Murashige And Skoog Medium
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