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Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
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Genetics
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JoVE Journal Genetics
Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

English

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7,411 Views

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11:12 min

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September 11, 2017

DOI:

10.3791/55946-v

DOI:
10.3791/55946-v

•

11:12 min
September 11, 2017

•

8 Views
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1Department of Biology, Section for Functional Genomics and Center for Bacterial Stress Response and Persistence (BASP),University of Copenhagen, 2Department of Microbiology and Infection Control,Statens Serum Institut

Summary

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Here, the power of a transposon-mediated random insertion of a non-coding DNA element was used to resolve its optimal chromosomal position.

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Frimodt-Møller, J., Charbon, G., Krogfelt, K. A., Løbner-Olesen, A. Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach. J. Vis. Exp. (127), e55946, doi:10.3791/55946 (2017).

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