Genetic Mapping of Thermotolerance Differences Between Species of <em>Saccharomyces</em> Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Carly V. Weiss; Julie N. Chuong; Rachel B. Brem

doi:10.3791/59972

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Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

JoVE Journal
Genetics

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JoVE Journal Genetics

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

15,858 Views

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10:08 min

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August 12, 2019

DOI:

10.3791/59972-v

DOI:

10.3791/59972-v

•

10:08 min

August 12, 2019

•

108 Views

Carly V. Weiss^1,2, Julie N. Chuong³, Rachel B. Brem^1,3

¹Department of Plant and Microbial Biology,University of California Berkeley, ²Department of Biology,Stanford University, ³Buck Institute for Research on Aging

Summary

Reciprocal hemizygosity via sequencing (RH-seq) is a powerful new method to map the genetic basis of a trait difference between species. Pools of hemizygotes are generated by transposon mutagenesis and their fitness is tracked through competitive growth using high-throughout sequencing. Analysis of the resulting data pinpoints genes underlying the trait.

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Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

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Summary

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