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Primer ınsan hava yolu epitel hücrelerinin hava yolu yüzeyi sıvı pH 'Sı gerçek zamanlı, yarı otomatik floresan ölçümü
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June 13, 2019
DOI:
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences,Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences,Newcastle University, 3Paediatric Respiratory Medicine, Great North Children’s Hospital,Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co
Summary
Automatically generated
Biz bir plaka okuyucu kullanarak ince film koşullarında hava yolu yüzeyi sıvı pH dinamik ölçümler yapmak için bir protokol sunuyoruz.
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Cite this Article
Saint-Criq, V., Haq, I. J., Gardner, A. I., Garnett, J. P., Ward, C., Brodlie, M., Gray, M. A. Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells. J. Vis. Exp. (148), e59815, doi:10.3791/59815 (2019).
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