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Journal
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Biochemistry
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一次人气道上皮细胞气道表面液体 pH 值的实时、半自动荧光测量
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
一次人气道上皮细胞气道表面液体 pH 值的实时、半自动荧光测量
DOI:
10.3791/59815-v
•
10:18 min
•
June 13, 2019
•
Vinciane Saint-Criq
,
Iram J. Haq
3
,
Aaron I. Gardner
,
James P. Garnett
4
,
Christopher Ward
2
,
Malcolm Brodlie
3
,
Michael A. Gray
1
Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences
,
Newcastle University
,
2
Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences
,
Newcastle University
,
3
Paediatric Respiratory Medicine, Great North Children’s Hospital
,
Newcastle upon Tyne Hospitals NHS Foundation Trust
,
4
Boehringer Ingelheim Pharma GmbH & Co
Chapters
00:04
Title
01:09
Primary Human Airway Epithelial Cell (hAEC) Preparation
01:53
Background Measurement
04:43
Kinetics Measurement
05:45
In Situ
pH Calibration
06:28
Data Analysis
07:58
Results: Representative pH Calibration in Primary hAECs
In Situ
09:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个协议, 以进行动态测量气道表面液体 ph 值在薄膜条件下使用板式读取器。
Tags
Real-time
Semi-automated
Fluorescent Measurement
Airway Surface Liquid PH
Primary Human Airway Epithelial Cells
Ion Channels
Transporters
Extracellular PH Regulation
Cystic Fibrosis
Asthma
COPD
Air-liquid Interface
Bicarbonate Buffer
Plate Reader
Temperature Control
Carbon Dioxide Control
Humidity Cassette
Kinetic Loop
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