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Real-Time, halbautomatisierte Fluoreszenzmessung der Luftbogenoberfläche Liquid pH der primären menschlichen Luftweg-Epithelzellen
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10:18 min
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June 13, 2019
DOI:
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences,Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences,Newcastle University, 3Paediatric Respiratory Medicine, Great North Children’s Hospital,Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co
Summary
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Wir stellen ein Protokoll vor, um dynamische Messungen der Atemwegsflüssigkeit pH unter Dünnschichtbedingungen mit einem Plattenleser zu machen.
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Cite this Article
Saint-Criq, V., Haq, I. J., Gardner, A. I., Garnett, J. P., Ward, C., Brodlie, M., Gray, M. A. Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells. J. Vis. Exp. (148), e59815, doi:10.3791/59815 (2019).
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