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Journal
/
Biochemistry
/
初代ヒト気道上皮細胞の気道表面液 pH をリアルタイムで半自動で蛍光測定
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
初代ヒト気道上皮細胞の気道表面液 pH をリアルタイムで半自動で蛍光測定
DOI:
10.3791/59815-v
•
10:18 min
•
June 13, 2019
•
Vinciane Saint-Criq
,
Iram J. Haq
3
,
Aaron I. Gardner
,
James P. Garnett
4
,
Christopher Ward
2
,
Malcolm Brodlie
3
,
Michael A. Gray
1
Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences
,
Newcastle University
,
2
Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences
,
Newcastle University
,
3
Paediatric Respiratory Medicine, Great North Children’s Hospital
,
Newcastle upon Tyne Hospitals NHS Foundation Trust
,
4
Boehringer Ingelheim Pharma GmbH & Co
Chapters
00:04
Title
01:09
Primary Human Airway Epithelial Cell (hAEC) Preparation
01:53
Background Measurement
04:43
Kinetics Measurement
05:45
In Situ
pH Calibration
06:28
Data Analysis
07:58
Results: Representative pH Calibration in Primary hAECs
In Situ
09:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々は、プレートリーダーを用いて、薄膜状態下で気道表面液 pH の動的測定を行うためのプロトコールを提示する。
Tags
Real-time
Semi-automated
Fluorescent Measurement
Airway Surface Liquid PH
Primary Human Airway Epithelial Cells
Ion Channels
Transporters
Extracellular PH Regulation
Cystic Fibrosis
Asthma
COPD
Air-liquid Interface
Bicarbonate Buffer
Plate Reader
Temperature Control
Carbon Dioxide Control
Humidity Cassette
Kinetic Loop
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