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Mesure fluorescente semi-automatisée en temps réel du pH liquide de la surface des voies respiratoires des cellules épithéliales des voies respiratoires primaires de l’homme
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10:18 min
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June 13, 2019
DOI:
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences,Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences,Newcastle University, 3Paediatric Respiratory Medicine, Great North Children’s Hospital,Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co
Summary
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Nous présentons un protocole pour faire des mesures dynamiques de la surface des voies respiratoires pH liquide dans des conditions de film mince à l’aide d’un lecteur de plaque.
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Cite this Article
Saint-Criq, V., Haq, I. J., Gardner, A. I., Garnett, J. P., Ward, C., Brodlie, M., Gray, M. A. Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells. J. Vis. Exp. (148), e59815, doi:10.3791/59815 (2019).
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